How do you manufacture recombinant proteins?

How do you manufacture recombinant proteins? Basic steps to get recombinant Protein: Amplification of gene of interest. Insert into cloning vector. Sub cloning into expression vector. Transformation into protein expressing host (bacteria (E coli), yeast, mammalian cells or baculovirus-insect cell system). How do you purify recombinant protein? The most widely Read more…

Are Photobucket pictures private?

Are Photobucket pictures private? Photobucket handles privacy levels differently than other photo sharing services like Flickr or Facebook. Instead of setting a privacy level at the individual photo, you set the privacy level (“Everyone,” “Private,” or “Password-protected”) at the album level. Is Photobucket still legit? The company was originally acquired Read more…