Does the presence of the mecA gene confirm MRSA?
Molecular amplification of the mecA gene is recognized as a benchmark to diagnose MRSA in the community as these genes are highly conserved among staphylococcal species [6].
What is mecA on blood culture?
In most methicillin-resistant staphylococci, the mecA gene is localized on the bacterial chromosome. The mecA gene encodes a penicillin binding protein (PBP2a) which has a low affinity for β-lactams (4, 16).
How is the mecA gene involved in the mechanism of resistance?
The mecA determinant encodes an extra penicillin-binding protein (PBP2a). The expression of resistance is achieved by a slow rate of acylation of PBP2a as well as a low affinity of the enzyme for β-lactams [6].
What resistance is mediated by the mecA gene?
Methicillin-resistant Staph.
Methicillin-resistant Staph. MRSA produces a penicillin binding protein 2a (mediated through the mecA gene), which is carried on the staphylococcal cassette chromosome mec (SCCmec) of which there are at least six different types recognized, and this results in resistance to all beta-lactam antibiotics.
What does mecA stand for?
MECA
| Acronym | Definition |
|---|---|
| MECA | Middle East Children’s Alliance |
| MECA | Maritime Electronic Commerce Association |
| MECA | Manufacturers of Emission Controls Association (Washington, DC) |
| MECA | Mars Environmental Compatibility Assessment (NASA JPL) |
What is methicillin used for?
methicillin, also called meticillin, antibiotic formerly used in the treatment of bacterial infections caused by organisms of the genus Staphylococcus. Methicillin is a semisynthetic derivative of penicillin.
How do you test for mecA gene?
Nucleic acid amplification tests, such as the polymerase chain reaction (PCR), can be used for direct detection of mecA, the most common gene mediating oxacillin resistance in staphylococci.
How many mec genes are there?
So far, six classes of mec gene complexes have been described (A, B, C1, C2, D, and E) in S.
Where did the mecA gene come from?
fleurettii mecA gene and its surrounding chromosomal region. Our finding suggests that SCCmec was generated in Staphylococcus cells living in animals by acquiring the intrinsic mecA region of S. fleurettii, which is a commensal bacterium of animals.
Where is mecA gene located?
And the mecA gene is the coding gene of PBP2′, and located in the SmaI fragment G of the chromosome map by Pattle P.A.,. A part of the structure of mecA is similar to that of the penicillinase gene.
What organisms did MRSA get mecA from?
MRSA produces a penicillin binding protein 2a (mediated through the mecA gene), which is carried on the staphylococcal cassette chromosome mec (SCCmec) of which there are at least six different types recognized, and this results in resistance to all beta-lactam antibiotics.
Why is methicillin not used?
Methicillin. Methicillin was the first semisynthetic penicillinase-resistant penicillin. It has been withdrawn from the market in the United States because of the high incidence of interstitial nephritis associated with its use.