What does T cell clonality mean?

The term is used to describe the early steps in tumorigenesis; in later stages of tumor growth and progression, monoclonal tumor cells may acquire a mutator phenotype, rendering the genome unstable.

What is the purpose of the clonality test?

A clonality test for immunoglobulin (IG) and T cell receptor (TCR) is a useful adjunctive method for the diagnosis of lymphoproliferative diseases (LPDs). Recently, the BIOMED-2 multiplex polymerase chain reaction (PCR) assay has been established as a standard method for assessing the clonality of LPDs.

What does a clonal T cell population mean?

Conditions such as autoimmune disorders, certain infections, immune suppression, and immune deficiencies are sometimes associated with small clonal T-cell populations. This means that one or more groups of cloned T-cells may be present in a person’s lymphocyte population without it being considered a lymphoma.

What is T cell clonality by PCR?

T-cell Clonality Panel (TCRB, TCRG), PCR – This test, which interrogates both the T-cell receptor beta (TCRB) locus and the T-cell receptor gamma (TCRG) locus by a PCR method based on the BIOMED-2 consensus, is useful in patients with suspected T-cell malignancies or to evaluate for residual disease after treatment.

How do you check clonality?

Currently, a number of different approaches can be used to detect clonality, including Southern blotting analyses, polymerase chain reaction (PCR) assays, and most recently, high-throughput sequencing [or next-generation sequencing (NGS)]; see Table 1.

What does B cell clonality mean?

Clonality analysis of B-cell populations can aid in the diagnosis of lymphoproliferative disorders. This test is used to support or refute the diagnosis of B-cell lymphomas/leukemias.

What is the meaning of clonality?

Clonality implies the state of a cell or a substance being derived from one source or the other. Thus there are terms like polyclonal—derived from many clones; oligoclonal—derived from a few clones; and monoclonal—derived from one clone. These terms are most commonly used in context of antibodies or immunocytes.

What is T-cell leukemia?

T-cell prolymphocytic leukemia (T-PLL) is an extremely rare and typically aggressive malignancy (cancer) that is characterized by the out of control growth of mature T-cells (T-lymphocytes). T-cells are a type of white blood cell that protects the body from infections.

What is Subclonal?

Subclonal mutation: mutation that is present in a subset of tumor cells in a tumor sample or biopsy. Subclone: a clone that is a descendant of the MRCA of the tumor sample, i.e. with associated CCF<1 in at least one region.

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