How do you do Alamar blue assay?
Prepare fresh cell culture medium and add alamarBlue® in an amount equal to 10% of the total volume. Mix the alamarBlue® reagent by shaking. Add 200 μL of alamarBlue® -containing medium mix to each well. Incubate spheroid cell cultures for 24 h at 37 °C protected from light.
How is Alamar blue calculated?
O1 = molar extinction coefficient (E) of oxidized alamarBlue (blue) at 570 nm* O2 = E of oxidized alamarBlue at 600 nm* A1 = absorbance of test wells at 570 nm. A2 = absorbance of test wells at 600 nm….62%
| Wavelength | Reduced (R) | Oxidized (O) |
|---|---|---|
| 600 nm | 14652 | 117216 |
| 630 nm | 5494 | 34798 |
What type of assay is Alamar Blue?
cell viability assay reagent
alamarBlue is a cell viability assay reagent which contains the cell permeable, non-toxic, and weakly fluorescent blue indicator dye called resazurin. This is a trusted and established reagent which has been available since 1993.
How does the Alamar blue assay measure cell viability?
AlamarBlue Cell Viability Reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change color in response to the chemical reduction due to cell growth.
Does Alamar blue expire?
alamarBlue® should be stored in the dark, since the compound is light sensitive (Table 2). The product may be stored for 12 months at room temperature. This expiration date is given on the product label. If shelf life beyond 12 months is desired, storage at 2-8°C increases shelf life to 20 months.
What is Presto blue?
PrestoBlue™ is a ready to use cell permeable resazurin-based solution that functions as a cell viability indicator by using the reducing power of living cells to quantitatively measure the proliferation of cells.
How do you dilute Alamar Blue?
Method
- Pipette 0.4 ml of alamarBlue into a test tube.
- Dilute to 10 mL with phosphate buffer.
- Mix thoroughly.
- Pipette 100 µL into each well of a clear, flat bottom microblate.
- Read absorbance at appropriate wavelengths.
What is microplate Alamar blue assay?
The 96-well Microplate Alamar Blue Assay (MABA) allows for the quantitative determination of drug susceptibility against any strain of replicating Mycobacterium tuberculosis to be completed within a week at minimal cost. The Low-Oxygen Recovery Assay (LORA) uses a recombinant M.
Is Alamar blue light sensitive?
alamarBlue® should be stored in the dark, since the compound is light sensitive (Table 2). The product may be stored for 12 months at room temperature.