What is Triton reagent?
General description. Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent.
What is a Triton in biology?
Triton X-100 is a commonly used detergent in laboratories. Triton X-100 is widely used to lyse cells to extract protein or organelles, or to permeabilize the membranes of living cells.
What does TX 100 do?
Triton X-100 (TX100) is one of the most widely used nonionic surfactants for lysing cells to extract protein and other cellular organelles or to permeabilize the living cell membrane for transfection (1–3).
Who makes Triton x100?
Triton X-100 was a registered trademark formerly owned by Rohm and Haas Co., but now owned by Union Carbide. GENERAL INFORMATION: X-100 is a nonionic detergent, 100% active ingredient, which is often used in bio- chemical applications to solubilize proteins.
Is Triton toxic?
Triton X-100 Solution (Part No. 310805): One 15 mL bottle containing 5% Triton X-100 in TBS. Hazard Statements: Harmful if swallowed (H302), Causes serious eye irritation (H319), Toxic to aquatic life with long lasting effects (H411).
Is Triton flammable?
Condition of flammability: Not flammable or combustible. Suitable extinguishing media: Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.
How do you make Triton?
Stock solution 10% (wt/vol) Triton X-100 solution i) Dissolve 10 g of Triton X-100 (e.g., Nacalai Tesque, Code 355-01 or similar grade) in 80 ml of Milli-Q water by stirring. ii) Add Milli-Q water to make 100 ml and stir until well mixed. iii) Store at 4 ºC.
Does Triton denature proteins?
Triton-X100 can denature proteins. It is typically used to clean up inclusion bodies (get rid of membrane proteins etc.).
Does NP-40 lysis nuclei?
NP-40 and Triton X-100 will not lyse nuclear membranes. After lysis, pellet the nuclei by centrifugation and transfer the supernatant to a new tube. If you wish to isolate both the nuclear and soluble fractions, resuspend the nuclear pellet in RIPA buffer.